#0263: HPV 59 virus-producing cell line for drug development
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TECH FIELD(S)
Research Tools
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FEATURES
Dr. Darron Brown has created an immortalized keratinocyte cell line containing HPV 59 that is able to produce infectious virus particles at early passage when grown in a simple culture system. These cells contain episomal HPV 59. The ERIN59 cell line, when induced to differentiate, express all known transcripts of the HPV 59 virus including the L1 major capsid gene, and the E6 and E7 oncogenes. No other cell lines have been shown to express the L1 major capsid protein in a simple cell culture system.
The cells at later passage contain integrated HPV 59, and express the E6 and E7 genes in abundance. Thus, the ERIN59 cell line can be utilized to study HPV 59 oncogenesis as well as the entire growth cycle of the virus. This cell line can ultimately be used in the development of drugs for the treatment of cervical cancer and other medical conditions that can result from an HPV infection.
In conclusion, the ERIN59 cell line is ideal for large scale and high throughput screening of antiviral and anti-cancer agents of all kinds, including small molecules, siRNAs, and others. The cells do not require growth in raft cultures, and are extremely easy to handle.
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BENEFITS
- Produces live HPV 59
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INVENTOR(S)
Dr. Darron Brown
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INTELLECTUAL PROPERTY STATUS
Non-patented
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CONTACT INFORMATION
For additional information on this technology, please contact Kun Ma, Business Development Manager, IURTC:
Indiana University Research & Technology Corporation
351 West 10th Street
Indianapolis, IN 46202
Telephone: 317.278.1958
kunma@iu.edu
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