#05061: Fast, Miniaturized, and Small-Scale Permethylation of Oligosaccharides
Miniaturized procedure improving the sensitivity for subsequent GC, LC and MS analysis
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TECH FIELD(S)
Technical procedure
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FEATURES
The analysis of methylated derivatives is treated as very useful in MS analyses. A widespread procedure for analysis of oligosaccharides is an in-solution permethylation approach. However, many disadvantages are associated with this procedure such as excessive sample handling, multi-step procedure, oxidative degradation and peeling reactions.
This invention utilizes a solid phase permethlyation approach, which overcomes these limitations and increases the sensitivity for subsequent analysis by GC, LC and MS. For a description of the inventions, see the inventor's web site: http://www.indiana.edu/~novweb/?page=glycomics.
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BENEFITS
- Eliminates excessive sample handing or sample degradation
- Avoids peeling reactions or oxidative degradation of glycans
- Allows on-line permethylation of glycan structures
- Effective for the analysis of glycans, allowing coupling of on-line permethylation to reversed-phase chromatography for separation
INVENTOR(S)
Pilsoo Kang - Indiana University, Department of Chemistry
Yehia Mechref, PhD - Indiana University, Department of Chemistry
Milos V. Novotny, PhD - Indiana University, Department of Chemistry
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INTELLECTUAL PROPERTY STATUS
US and foreign patents pending
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CONTACT INFORMATION
For additional information on this technology, please contact Bill Brizzard, Director of Technology Transfer , IURTC:
Indiana University Research & Technology Corporation
501 North Morton Street, Suite 204
Bloomington, IN 47404
Telephone:812-855-3597
bbrizzar@iu.edu
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